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The ISO-Intense regime is an internationally standardized version of the smoking regime introduced in 1999 by Health Canada to compensate for potential blocking of the filter ventilation holes during smoking and to reflect the larger puff volumes taken by many smokers

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Abbreviations ALI: Airliquid interface BALF: Bronchoalveolar lavage fluid BK: Large conductance, Ca 2+ -activated, and voltage-dependent K + CBF: Ciliary beat frequency CFTR: Cystic fibrosis transmembrane conductance regulator COPD: Chronic obstructive pulmonary disease e-cig: E-cigarette FOXJ1: Forkhead box protein J1 HBEC: Human bronchial epithelial cells IL-6: Interleukin-6 IL-8: Interleukin-8 KRT5: Keratin 5 MMP-9: Matrix metalloproteinase-9 MUC5AC: Mucin 5AC MUC5B: Mucin 5B PG: Propylene glycol SCGB1A1: Secretoglobin family 1A member 1 TGFB1: Transforming growth factor-beta1 VG: Vegetable glycerin References Jerzynski, T., Stimson, G

PMC 12121063