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Acute paraxanthine ingestion improves cognition and short-term memory and helps sustain attention in a double-blind, placebo-controlled, crossover trial

The cycle threshold (CT) for the target genes ( SIRT-1 and CYP2E1 ) and the endogenous control GAPDH for each sample were detected and the relative quantitation of SIRT-1 and CYP2E1 mRNA expression levels was estimated by normalizing CT of each gene with the CT of GAPDH in each sample using the 2 CT method (Livak and Schmittgen, 2001)

PubMed CrossRef Marso SP, Bain SC, Consoli A, et al

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The major types of fistulas are colovesical fistulas (65 percent) and colovaginal fistulas (25 percent), followed by coloenteric and colouterine fistulas