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A prior study from our laboratory demonstrated that 6-month inhalation of E-CIG vapor containing nicotine (24 mg/ ml) led to down-regulation of both cystine/glutamate antiporter (xCT) and glutamate transporter-1 (GLT-1) in the STR of outbred female CD1 mice (Sari et al., 2016)
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I dont suggest using a used one because it may be quite dirty and difficult to clean thoroughly
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