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In addition, they used a more aggressive puffing regimen (50 puffs/sample) as well as DNPH impingers for derivatization and HPLC analysis 4

Despite some inconsistencies, particularly among studies assessing respiratory disorders and suffering from methodological limitations and possible biases 30-33 , the findings from the reviewed studies indicate a growing body of evidence supporting the harmful effects of SHS on pets, with reported associations ranging from respiratory and cardiovascular alterations to neoplasms and biochemical markers of oxidative stress and inflammation

Graphical images on cigarette packets have also been introduced, among other initiatives such as taxes, to persuade workers not to smoke