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Shaking can cause foaming, which indicates that the peptide chains are breaking and the compound is losing its efficacy for your metabolic assays

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The main purpose of the current paper is to study the oxidative stress state in children with chronic hepatitis by using indirect approach in which antioxidant enzymes such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT) are determined in the liver tissue