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Using 0.1 M sodium phosphate buffer (pH 7.4) with phenylmethylsulfonyl fluoride (PMSF) as a protease inhibitor, skin tissue samples were homogenized at 4000 rpm x g for 10 min at 4 C and then centrifuged for atleast 30 mins and then the resultant supernatant was collected

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This reduction correlates directly with slower wound healing, visible skin aging, and yes, hair thinning